Porcine GPX1(Glutathione peroxidase 1) ELISA Kit

GPX1(Glutathione peroxidase 1) Basic information
GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead.[clarification needed] The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

Porcine GPX1(Glutathione peroxidase 1) ELISA Kit test method
Feiyue’s Porcine GPX1(Glutathione peroxidase 1) is an ELISA reagent for detection of GPX1(Glutathione peroxidase 1) in Serum, plasma, tissue homogenates and other biological fluids.
This kit uses sandwich ELISA to detect the concentration of Porcine GPX1(Glutathione peroxidase 1). Porcine GPX1(Glutathione peroxidase 1)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Porcine GPX1(Glutathione peroxidase 1) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Porcine GPX1(Glutathione peroxidase 1) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

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Контактное лицо: Agnes