Human IL-16(Interleukin 16) ELISA Kit

Catalog NO.: FY-EH4723
Reactivity: Human
Assay range: 15.6-1,000pg/mL
Sensitivity：＜ 5.6pg/mL
Detection Method：Sandwich ELISA, Double Antibody
Size：48T, 96T
Storage：2-8 ℃ for 6 months
CV （%）：Intra-Assay: CV<8%；Inter-Assay: CV<10%
Note：For Research Use Only

IL-16(Interleukin 16)  Basic information
Pro-interleukin-16 is a protein that in humans is encoded by the IL16 gene.This gene was discovered in 1982 at Boston University by Dr. David Center and Dr. William Cruikshank.
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Two alternatively spliced transcript variants encoding distinct isoforms have been reported.

Human IL-16(Interleukin 16) ELISA Kit test method
Feiyue’s Human IL-16(Interleukin 16) Elisa kit is an ELISA reagent for detection of IL-16(Interleukin 16) in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.This kit uses sandwich ELISA to detect the concentration of  IL-16(Interleukin 16) .  IL-16(Interleukin 16) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- IL-16(Interleukin 16)  antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of  IL-16(Interleukin 16)  in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

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