Human IL-6 ELISA Kits

Catalog NO.: FY-EH3991

The ELISA kit is a competitive enzyme immunoassay technique for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensitivity: 1.88 pg/ml.

Detection Range: 3.13-200 pg/ml

Specificity: This kit recognizes spermidine in samples.
No significant cross-reactivity or interference between spermidine and analogues was observed.

Repeatability: Coefficient of variation is < 10%.

Test principle：
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated.
Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.

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