Bovine Catalase (CAT) ELISA Kit

Bovine CAT(Catalase) ELISA Kit Basic Information 
Feiyue's Bovine CAT(Catalase) ELISA Kit is an ELISA reagent for detection of CAT(Catalase), plasma or cell with sensitivity, specificity and consistency.

CAT(Catalase) Introduction
Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a very important enzyme in protecting the cell from oxidative damage by reactive oxygen species (ROS). Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Bovine CAT(Catalase) ELISA Kit Test method
This ELISA kit uses the Sandwich-ELISA principle. The micro plate provided in this kit has been pre-coated with an antibody specific to Bovine CAT. Standards or samples and Horseradish Peroxidase (HRP) labeled detection antibody specific for Bovine CAT are added to the plate wells together and incubated. After washing off unbound material, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Bovine CAT in the samples is then calculated from the OD value by establishing a standard curve.