Bovine HSP90(Heat shock protein 90) ELISA Kit

HSP90(Heat shock protein 90) Basic information
Hsp90 (heat shock protein 90) is a chaperone protein that assists other proteins to fold properly, stabilizes proteins against heat stress, and aids in protein degradation. It also stabilizes a number of proteins required for tumor growth, which is why Hsp90 inhibitors are investigated as anti-cancer drugs.
Heat shock proteins, as a class, are among the most highly expressed cellular proteins across all species. As their name implies, heat shock proteins protect cells when stressed by elevated temperatures. They account for 1–2% of total protein in unstressed cells. However, when cells are heated, the fraction of heat shock proteins increases to 4–6% of cellular proteins.
Heat shock protein 90 (Hsp90) is one of the most common of the heat-related proteins. The «90» comes from the fact that it has a mass of roughly 90 kilodaltons. A 90 kDa protein is considered fairly large for a non-fibrous protein. Hsp90 is found in bacteria and all branches of eukarya, but it is apparently absent in archaea. Whereas cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions.
This protein was first isolated by extracting proteins from cells stressed by heating, dehydrating or by other means, all of which caused the cell's proteins to begin to denature. However it was later discovered that Hsp90 also has essential functions in unstressed cells.
 
 
Bovine HSP90(Heat shock protein 90) ELISA Kit test method
Feiyue’s Bovine HSP90(Heat shock protein 90) is an ELISA reagent for detection of HSP90(Heat shock protein 90) in Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
This kit uses sandwich ELISA to detect the concentration of Bovine HSP90(Heat shock protein 90). Bovine HSP90(Heat shock protein 90)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Bovine HSP90(Heat shock protein 90) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Bovine HSP90(Heat shock protein 90) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

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Контактна особа: Agnes