STAT3(Signal transducer and activator of transcription 3) Basic information
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor which in humans is encoded by the STAT3 gene. It is a member of the STAT protein family.
Human STAT3(Signal transducer and activator of transcription 3) ELISA Kit test method
Feiyue’s Human STAT3(Signal transducer and activator of transcription 3) Elisa kit is an ELISA reagent for detection of STAT3 in Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.This kit uses sandwich ELISA to detect the concentration of STAT3 . STAT3 -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti- STAT3(Signal transducer and activator of transcription 3) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of STAT3 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
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