Rat PCT(Procalcitonin) ELISA Kit

Rat PCT(Procalcitonin) ELISA Kit Basic Information 
Product Name: Rat PCT(Procalcitonin) ELISA Kit
Catalog NO.: FY-ER4188
Alias: PCT; Procalcitonin
Application: PCT ELISA Kit allows for the in vitro quantitative determination of PCT concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
Sensitivity: <9.375pg/ml
Species: Rat
CV %（）
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
Feiyue’s Rat PCT is an ELISA reagent for detection of Canine PCT in serum, plasma or cell with sensitivity, specificity and consistency.

Rat PCT(Procalcitonin) Introduciton
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It was first identified by Leonard J. Deftos and Bernard A. Roos in the 1970s. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a pro-inflammatory stimulus, especially of bacterial origin. It is therefore often classed as an acute phase reactant.

Rat PCT(Procalcitonin) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Rat PCT.
Rat PCT-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat PCT antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rat PCT in the sample can be calculated by comparing the absorbance of the sample with the standard curve.