Canine PCT(procalcitonin) ELISA Kit

Canine PCT(procalcitonin) ELISA Kit Basic Information 
Product Name: Canine PCT(procalcitonin) ELISA Kit
Catalog NO.: FY-EC4186
Alias: PCT; Procalcitonin
Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
Sensitivity: <18.75pg/ml
Species: Canine
UniProt ID: P41547
CV %（）
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
 
Feiyue’s Canine PCT is an ELISA reagent for detection of Canine PCT in serum, plasma or cell with sensitivity, specificity and consistency.

Canine PCT(procalcitonin) Introduciton
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It was first identified by Leonard J. Deftos and Bernard A. Roos in the 1970s. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a pro-inflammatory stimulus, especially of bacterial origin. It is therefore often classed as an acute phase reactant.

Canine PCT(procalcitonin) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Canine PCT.
Canine PCT-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Canine PCT antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Canine PCT in the sample can be calculated by comparing the absorbance of the sample with the standard curve.