Rat PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit

Rat PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit Basic Information 
Product Name: Rat PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit
Catalog NO.: FY-ER4182
Alias: Serpine1; PAI-1; PAI1; SERPINE1; Nexin; PLANH1; Endothelial plasminogen activator inhibitor; PAISerpin E1; PLANH1; plasminogen activator inhibitor 1; serine; or cysteine proteinase inhibitor; clade E; nexin; plasminogenactivator inhibitor type 1; member 1; serpin peptidase inhibitor; clade E; nexin; plasminogen activator inhibitortype 1; member 1
Application: PAI-1 ELISA Kit allows for the in vitro quantitative determination of PAI-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
Sensitivity: <0.188ng/ml
Species: Rat
UniProt ID: P20961
CV %（）
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
 
Feiyue’s Rat PAI-1 is an ELISA reagent for detection of PAI-1 in serum, plasma or cell with sensitivity, specificity and consistency.

Rat PAI-1(Plasminogen Activator Inhibitor 1) Introduciton
Plasminogen activator inhibitor-1 (PAI-1) also known as endothelial plasminogen activator inhibitor or serpin E1 is a protein that in humans is encoded by the SERPINE1 gene. Elevated PAI-1 is a risk factor for thrombosis and atherosclerosis. PAI-1 is a serine protease inhibitor (serpin) that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots). It is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1's main function entails the inhibition of urokinase plasminogen activator (uPA), an enzyme responsible for the cleavage of plasminogen to form plasmin. 

Rat PAI-1(Plasminogen Activator Inhibitor 1) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Rat PAI-1.
Rat PAI-1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rat PAI-1 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rat PAI-1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.