Feiyuebio professional Customize Service

Feiyuebio Customize Service： ELISA kit
Lead time3-5 days
SpecyCustomer's sample
Range
According to customer's requirements

SensitivityAccording to customer's requirements
NotesWe can design and produce your required ELISA Kits for a wide range of targets and species. Just submit your detailed required information, Custom ELISA Kit service will be provided whout any risk.

1, Project communication
Well know about the project background and expected goals to determine the development plan. Specific ELISA kits can be developed according to your individual needs. The content of the target substance can be quantitatively detected by ELISA sandwich method, competitive method (antigen determination), indirect method (antibody determination) or competitive method (small molecule compound determination) .
2, Immunogen preparation
a. Analyze the gene and amino acid sequence of the target protein, carry out antigenicity and expression analysis, codon optimization, and complete the whole gene synthesis.
b. Construct prokaryotic expression vector of recombinant protein.
c. Transform the E. Coli system, conduct recombinant protein expression induction and nickel column affinity purification to obtain 1-3 mg of purified recombinant protein with a purity of >85%.
d. Preparation of immunogens for animal immunization. For small target, molecular modification through structural analysis and coupling to carrier protein for immunization and detection.
3, Antibody design
Antibodie types need to prepare are determined according to the project communication. Generally, sandwich ELISA requires antibodies of different epitopes for pairing. It is recommended to prepare polyclonal antibodies and monoclonal antibodies at the same time. Ensure efficient pairing for the highest sensitivity. Other types of ELISA are selected according to detailed situation.
4, ELISA kit production