sRAGE(soluble Receptor for Advanced Glycation End product) Basic information
RAGE (receptor for advanced glycation endproducts), also called AGER, is a 35 kilodalton transmembrane receptor of the immunoglobulin super family which was first characterized in 1992 by Neeper et al. Its name comes from its ability to bind advanced glycation endproducts (AGE), which include chiefly glycoproteins, the glycans of which have been modified non-enzymatically through the Maillard reaction. In view of its inflammatory function in innate immunity and its ability to detect a class of ligands through a common structural motif, RAGE is often referred to as a pattern recognition receptor. RAGE also has at least one other agonistic ligand: high mobility group protein B1 (HMGB1). HMGB1 is an intracellular DNA-binding protein important in chromatin remodeling which can be released by necrotic cells passively, and by active secretion from macrophages, natural killer cells, and dendritic cells.
Human sRAGE(soluble Receptor for Advanced Glycation End product) ELISA Kit test method
Feiyue’s Human sRAGE(soluble Receptor for Advanced Glycation End product) is an ELISA reagent for detection of sRAGE(soluble Receptor for Advanced Glycation End product) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
This kit uses sandwich ELISA to detect the concentration of Human sRAGE(soluble Receptor for Advanced Glycation End product). Human sRAGE(soluble Receptor for Advanced Glycation End product)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human sRAGE(soluble Receptor for Advanced Glycation End product) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human sRAGE(soluble Receptor for Advanced Glycation End product) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
This site uses "cookies". We do not share your data, they are used only for technical purposes and for the convenience of using the site (location, last selection, favorites). More details in Terms of use
