HSP70(Heat shock protein 70) Basic information
The 70 kilodalton heat shock proteins (Hsp70s or DnaK) are a family of conserved ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. Intracellularly localized Hsp70s are an important part of the cell's machinery for protein folding, performing chaperoning functions, and helping to protect cells from the adverse effects of physiological stresses. Additionally, membrane-bound Hsp70s have been identified as a potential target for cancer therapies and their extracellularly localized counterparts have been identified as having both membrane-bound and membrane-free structures.
Bovine HSP70(Heat shock protein 70) ELISA Kit test method
Feiyue’s Bovine HSP70(Heat shock protein 70) is an ELISA reagent for detection of HSP70(Heat shock protein 70) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
This kit uses sandwich ELISA to detect the concentration of Bovine HSP70(Heat shock protein 70). Bovine HSP70(Heat shock protein 70)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Bovine HSP70(Heat shock protein 70) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Bovine HSP70(Heat shock protein 70) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
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