Human GIP(Gastric inhibitory polypeptide) ELISA Kit

GIP(Gastric inhibitory polypeptide) Basic information
Gastric inhibitory polypeptide (GIP), or gastric inhibitory peptide is an inhibiting hormone of the secretin family of hormones. While it is weak inhibitor of gastric acid secretion, its main role is to stimulate insulin secretion.GIP, along with glucagon-like peptide-1 (GLP-1), belongs to a class of molecules referred to as incretins.

Human GIP(Gastric inhibitory polypeptide) ELISA Kit Test method
This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.

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