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LH(Luteinizing Hormone) Basic information
Luteinizing hormone (LH, also known as lutropin and sometimes lutrophin[1]) is a hormone produced by gonadotropic cells in the anterior pituitary gland. The production of LH is regulated by gonadotropin-releasing hormone (GnRH) from the hypothalamus. In females, an acute rise of LH («LH surge») triggers ovulation and development of the corpus luteum. In males, where LH had also been called interstitial cell–stimulating hormone (ICSH), it stimulates Leydig cell production of testosterone. It acts synergistically with follicle-stimulating hormone (FSH). In both males and females, LH works upon endocrine cells in the gonads to produce androgens.
Porcine LH(Luteinizing Hormone) ELISA Kit test method
Feiyue’s Porcine LH(Luteinizing Hormone) is an ELISA reagent for detection of LH(Luteinizing Hormone) in serum, plasma and other biological fluids.
This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.
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