Product name：Mouse Gpt(Alanine aminotransferase 1) ELISA Kit
Catalog NO.：FY-EM4537
Reactivity: Mouse
Assay range: 0.156-10ng/mL
Sensitivity: <0.057ng/mL
Detection Method: Double-antibody Sandwich
Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
CV (%): Intra-Assay: CV<8%; Inter-Assay: CV<10%
Note: For Research Use Only
Gpt(Alanine aminotransferase 1) Basic information Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called alanine aminotransferase (ALT or ALAT) and was formerly called serum glutamate-pyruvate transaminase or serum glutamic-pyruvic transaminase (SGPT) and was first characterized in the mid-1950s by Arthur Karmen and colleagues. ALT is found in plasma and in various body tissues but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
Mouse Gpt(Alanine aminotransferase 1) ELISA Kit test method Feiyue’s Mouse Gpt(Alanine aminotransferase 1) is an ELISA reagent for detection of Gpt(Alanine aminotransferase 1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
This kit uses sandwich ELISA to detect the concentration of Mouse Gpt(Alanine aminotransferase 1). Mouse Gpt(Alanine aminotransferase 1)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse Gpt(Alanine aminotransferase 1) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Mouse Gpt(Alanine aminotransferase 1) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
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