Human PARP1(Poly [ADP-ribose] polymerase 1) ELISA Kit

Human PARP1(Poly [ADP-ribose] polymerase 1) ELISA Kit Basic Information 
Product Name: Human PARP1(Poly [ADP-ribose] polymerase 1) ELISA Kit
Catalog NO.: FY-EH4183
Alias: PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; Poly[ADP-ribose] synthase 1; ADPRT 1
Application: PARP1 ELISA Kit allows for the in vitro quantitative determination of PARP1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
Sensitivity: <0.375ng/ml
Species: Human
UniProt ID: P09874
CV %（）
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
 
Feiyue’s Human PARP1 is an ELISA reagent for detection of PARP1 in serum, plasma or cell with sensitivity, specificity and consistency.

Human PARP1(Poly [ADP-ribose] polymerase 1) Introduciton
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. It is the most abundant of the PARP family of enzymes, accounting for 90% of the NAD+ used by the family. PARP1 is mostly present in cell nucleus, but cytosolic fraction of this protein was also reported. PARP1 acts as a first responder that detects DNA damage and then facilitates choice of repair pathway. PARP1 contributes to repair efficiency by ADP-ribosylation of histones leading to decompaction of chromatin structure, and by interacting with and modifying multiple DNA repair factors.

Human PARP1(Poly [ADP-ribose] polymerase 1) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Human PARP1.
Human PARP1-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human PARP1 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human PARP1 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.