PAF(Platelet Activating Factor) ELISA Kit

PAF(Platelet Activating Factor) ELISA Kit Basic Information 
Product Name: PAF(Platelet Activating Factor) ELISA Kit
Catalog NO.: FY-EU4181
Alias: Platelet Activating Factor
Size: 48T, 96T
Storage: 2-8 ℃ for 6 months
Sensitivity: <0.094ng/ml
Species: Universal
CV %（）
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note: For Research Use Only
 
Feiyue’s PAF is an ELISA reagent for detection of Human PAF in serum, plasma or cell with sensitivity, specificity and consistency.

PAF(Platelet Activating Factor) Introduciton
Platelet-activating factor, also known as PAF, PAF-acether or AGEPC (acetyl-glyceryl-ether-phosphorylcholine), is a potent phospholipid activator and mediator of many leukocyte functions, platelet aggregation and degranulation, inflammation, and anaphylaxis. It is also involved in changes to vascular permeability, the oxidative burst, chemotaxis of leukocytes, as well as augmentation of arachidonic acid metabolism in phagocytes. PAF is produced by a variety of cells, but especially those involved in host defense, such as platelets, endothelial cells, neutrophils, monocytes, and macrophages. PAF is continuously produced by these cells but in low quantities and production is controlled by the activity of PAF acetylhydrolases. It is produced in larger quantities by inflammatory cells in response to specific stimuli.

PAF(Platelet Activating Factor) ELISA Kit Test method
This kit was based on Competitive-ELISA detection method.
The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme -substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.