Bovine Interleukin 6 (IL-6) ELISA Kit

IL-6(Interleukin 6) Introduciton
IL-6 is secreted by macrophages in response to specific microbial molecules, referred to as pathogen-associated molecular patterns (PAMPs). These PAMPs bind to an important group of detection molecules of the innate immune system, called pattern recognition receptors (PRRs), including Toll-like receptors (TLRs). These are present on the cell surface and intracellular compartments and induce intracellular signaling cascades that give rise to inflammatory cytokine production. IL-6 is an important mediator of fever and of the acute phase response.
Bovine IL-6(Interleukin 6) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Bovine IL-6.
Bovine IL-6-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Bovine IL-6 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Bovine IL-6 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.