Bovine Glutathione peroxidase (GSH-Px) ELISA Kit

Bovine Glutathione peroxidase (GSH-Px) Introduction
GPX1 is an enzyme that in humans is encoded by the GPX1 gene on chromosome 3. This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides.
Bovine Glutathione peroxidase (GSH-Px) ELISA Kit Test Method
This ELISA kit uses the Sandwich-ELISA principle. The micro plate provided in this kit has been pre-coated with an antibody specific to Bovine GSH-Px. Standards or samples and Horseradish Peroxidase (HRP) labeled detection antibody specific for Bovine GSH-Px are added to the plate wells together and incubated. After washing off unbound material, TMB substrate solution is added to all wells and incubated. An enzyme-catalyzed reaction generates a blue color in the solution, thereafter, stop solution is added to stop the substrate reaction and the color turns yellow. The yellow solution is read at a wavelength of 450nm. The concentration of Bovine GSH-Px in the samples is then calculated from the OD value by establishing a standard curve.