Chicken Activin A (ACVA) ELISA kit

Chicken Activin A (ACVA) ELISA kit Basic Information 
Product Name
Chicken Activin A (ACVA) ELISA kit

Catalog NO.
FY-ECH4773

Alias
ACVA

Species
Chicken

Alternative Names
ACVA

Detection range
24-400pg/mL

Sensitivity 
＜12.6pg/mL

Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other samples

Test Method
Sandwich ELISA, Double Antibody

Available
48T, 96T

Storage
2-8℃ for 6 months

Note
For Research Use Only
Activin A (ACVA) Introduction
Activin and inhibin are two closely related protein complexes that have almost directly opposite biological effects. Identified in 1986, activin enhances FSH biosynthesis and secretion, and participates in the regulation of the menstrual cycle. Many other functions have been found to be exerted by activin, including roles in cell proliferation, differentiation, apoptosis, metabolism, homeostasis, immune response, wound repair,[4] and endocrine function. Conversely, inhibin downregulates FSH synthesis and inhibits FSH secretion. The existence of inhibin was hypothesized as early as 1916; however, it was not demonstrated to exist until Neena Schwartz and Cornelia Channing's work in the mid-1970s, after which both proteins were molecularly characterized ten years later.Activin is a dimer composed of two identical or very similar beta subunits. Inhibin is also a dimer wherein the first component is a beta subunit similar or identical to the beta subunit in activin. However, in contrast to activin, the second component of the inhibin dimer is a more distantly-related alpha subunit. Activin, inhibin and a number of other structurally related proteins such as anti-Müllerian hormone, bone morphogenetic protein, and growth differentiation factor belong to the TGF-β protein superfamily
Activin is produced in the gonads, pituitary gland, placenta, and other organs: In the ovarian follicle, activin increases FSH binding and FSH-induced aromatization. It participates in androgen synthesis enhancing LH action in the ovary and testis. In the male, activin enhances spermatogenesis. Activin is strongly expressed in wounded skin, and overexpression of activin in epidermis of transgenic mice improves wound healing and enhances scar formation. Its action in wound repair and skin morphogenesis is through stimulation of keratinocytes and stromal cells in a dose-dependent manner. Activin also regulates the morphogenesis of branching organs such as the prostate, lung, and especially kidney. Activin A increased the expression level of type-I collagen suggesting that activin A acts as a potent activator of fibroblasts. Lack of activin during development results in neural developmental defects. Upregulation of Activin A drives pluripotent stem cells into a mesoendodermal fate, and thus provides a useful tool for stem cell differentiation and organoid formation.
Chicken Activin A (ACVA) ELISA kit Test method
This kit uses sandwich ELISA to detect the concentration of Activin A (ACVA) The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A (ACVA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A (ACVA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain of Activin A (ACVA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A (ACVA) in the samples is then determined by comparing the OD of the samples to the standard curve.