Mouse ARO (Aromatase) Introduction
Aromatase is a key enzyme in estrogen biosynthesis , encoded by the gene CYP19A1 in humans, and a member of the cytochrome P450 superfamily. Aromatase is a monooxygenase that catalyzes the aromatization reaction from androgens to estrogens in steps involved in the synthesis of steroid hormones. Aromatase occurs in many human tissues, such as the gonads ( granulosa cells ), brain , adipose tissue , placenta , blood vessels , skin , and bones , and is important for the development of secondary sexual characteristics during puberty .
Mouse ARO (Aromatase) ELISA Kit Test Method
This kit uses sandwich ELISA to detect the concentration of Mouse ARO.
Mouse ARO-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Mouse ARO antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of Mouse ARO in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
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