Feiyuebio's Human APOA1(Apolipoprotein A-I) ELISA Kit is an ELISA reagent for detection of APOA1(Apolipoprotein A-I) , plasma or cell with sensitivity, specificity and consistency.
APOA1(Apolipoprotein A-I) Introduction
Apolipoprotein A1 is a protein that in humans is encoded by the APOA1 gene.As the major component of HDL particles, it has a specific role in lipid metabolism. The text in a 2014 report suggested that APOA1 mRNA is regulated by endogenously expressed antisense RNA.
Human APOA1(Apolipoprotein A-I) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of APOA1(Apolipoprotein A-I) .APOA1(Apolipoprotein A-I) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional APOA1(Apolipoprotein A-I) antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of APOA1(Apolipoprotein A-I) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
Reference:
1. GRCh38: Ensembl release 89: ENSG00000118137 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032083 - Ensembl, May 2017
3.«Human PubMed Reference:». National Center for Biotechnology Information, U.S. National Library of Medicine.
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